The novel proteins PAT and Barstar have been found to have no pathogenic properties and no biologically significant sequence similarities with any known toxins or allergens. PAT and Barstar were found to be rapidly digested as demonstrated by digestibility studies using simulated gastric fluid with pepsin. Assessors noted that PAT protein was not degraded after heat treatment (90°C at 60 min) as evidenced by the persistence of the resulting band from SDS-PAGE and Western bot. On the other hand, Barstar protein produced soluble oligomers after heat treatment which suggested that it is not stable at 95°C. Meanwhile, acute oral toxicity test results indicate that the No Observed Effect Levels (NOEL) for PAT and Barstar are >2000 mg/kg bodyweight and >1000 mg/kg bodyweight, respectively. These values cannot be realistically ingested, especially when considering the level of expression of PAT and Barstar in the plant tissues. The assessors additionally concurred that the processing of the oilseed rape grain into refined and food-grade oil will result to the degradation of all protein components. In view of these, the pathway to harm for PAT and Barstar cannot be realized. Therefore the overall risk for Canola RF3 is negligible to very minimal.